As test statistics for asexuality, we utilized the presence of duplicated multilocus genotypes and maximum deviation from random mating (F IS ) (53 –55). The ratio that is genotype-to-individualG N ratio) had been used to recognize multilocus genotypes (55) (Table S1). Individually developed lineages that are asexualclones) originating from split intimate activities had been distinguished from somewhat different multilocus genotypes that diversified through accumulation of mutations or scoring mistakes by determining the likelih d, p sex , after the methodology outlined in ref. 34 and applied in GENCLONE 2.0 (56). (more…)